- authors
- P. Mehrabi, E.-C. Schulz, M. Agthe, S. Horrell, G. Bourenkov, D. von Stetten, J.-P. Leimkohl, H. Schikora, T. R. Schneider, A. R. Pearson, F. Tellkamp, R. J. D. Miller.
- date published
- Sept. 16, 2019
- journal
- Nature Methods
- volume, number
- 10 (16)
- pages
- 979–982
- web page
- https://www.nature.com/articles/s41592-019-0553-1
- doi
- https://doi.org/10.1038/s41592-019-0553-1
- abstract
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.
